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Solutions Small Molecules Discovery Biology Cell and Protein Sciences Antibody Discovery

Antibody Discovery

Antibody Discovery services at Cell and Protein Sciences offers an integrated platform to help you identify and optimize high-affinity antibodies. Our state-of-the-art technology at Hyderabad and deep expertise empower you to develop antibodies with the precision and quality required for successful drug development. We utilize established technologies such as Hybridoma and Phage display platforms for monoclonal antibody discovery. Our facility harbors latest equipment to support the discovery and characterize discovered antibodies.

Hybridoma Technology

Developing high-affinity, target-specific monoclonal antibodies is essential for advancing research and development programs. Hybridoma technology is a reliable platform for discovering monoclonal antibodies targeting specific antigens. Cell and Protein Sciences at Aragen offers comprehensive services for antibody discovery using the hybridoma method, which includes antigen generation, immunization, identification, and purification of the best binding antibodies through a thoroughly tested pipeline. Additionally, we provide V-gene sequencing for the identified molecules.

Why Aragen?

  • State-of-the-art transgenic animal facility
  • Various immunization strategies
  • Early-stage screening using hybridoma supernatants
  • Cell-based screening using advance flow cytometers
  • SPR-based affinity screening
  • V-gene sequencing and antibody reformatting

Timeline: ~ 5 months

V-gene Sequencing

At Cell and Protein Sciences, we offer V-gene sequencing services that enable detailed analysis of the immune repertoire of hybridoma cells, driving forward innovative research in antibody discovery.

Key features of our V-gene sequencing services include:

  • Customizable sequencing options: Tailor your sequencing project to focus on specific V, D, and J gene segments
  • Data analysis: Bioinformatics databases such as VBASE2, IMGT for analyzing VH and VL; tools such as Geneious Prime and Sequencher for detailed insights into CDR sequence diversity.

Timelines: 2-3 weeks

Phage Display Technology

At Cell and Protein Sciences, we provide comprehensive phage display services, from library generation to lead candidate identification. Our services are tailored to meet the unique needs of each project, enabling precise and efficient antibody discovery. 

Our offerings include:

Custom library construction: We create highly diverse VHH and scFv libraries (107-1011 transformants) for broad antibody repertoires, catering to your specific research objectives, maximizing the chances of identifying lead candidates with desired binding characteristics

Biopanning: For enrichment of binders against a specific antigen, we offer two screening methodologies such as solid-phase (immunotubes based) and solution-sorting (bead based) screening

Screening: Using phage display platforms, we screen large libraries to identify molecules that exhibit strong binding affinity to target antigens, receptors, or other molecules of interest employing monoclonal phage ELISA and soluble protein ELISA

Timelines: Library Construction and Screening: 3 – 4 months